Elephant Endotheliotropic Herpesvirus (EEHV) is one of the leading causes of death for Asian elephant calves in human care and in the wild. This project is working towards the development of an effective vaccine against EEHV. Dr. Paul Ling of the Baylor College of Medicine recently identified several important features of EEHV that are important targets for T cell-mediated immunity which is critical for control of herpesvirus infections and another step towards finding a workable vaccine that will benefit all elephants. Antibody responses are also expected to contribute to protective immunity against lethal EEHV infection. Currently, simple EEHV antibody tests with high specificity and sensitivity are not available. To address this need, this project will use a novel approach known as the Luciferase Immunoprecipitation System (LIPS).